- Hilary Jardine - Susan
- Keeya King - Violet
- Nicole Muñoz - Jack
- Tricia Helfer - Dracula
- Tim Guinee - Ted
- David Cubitt - John
- John Cassini - Max Maddox
- Cory Gruter-Andrew - Troy Johnson
- Dustin MacDougall - Shade
- Toby Levins - Bosworth
- Nelson Wong - Nguyen
- Donny Lucas - Chad Johnson
- Cassidy Nugent - Tabby
- Tammy Gillis - Monica
- Marc Anthony Williams - Security Official
- Karina Frislev - Sister
- Adrian Petriw - Colin / Daywalker #1
- Sean Millington - Large Man
- Colleen Winton - Abigail
- Shawn Stewart - Loveland Guard
- Richard Harmon - Max Borman
- Holly Dignard - Jennifer
- Bradley Stryker - Matty
- Chris Ippolito - Vampire #1
- Michael Eklund - Jacob Van Helsing
- Michael Eklund - Abraham Van Helsing
- Ryan Jinn - Lee
- C. Ernst Harth - Heavy Set Guard
- Julie Lynn-Mortensen - Lillian Van Helsing
- Adrian Hough - Edward Hawkins
- Tony Alcantar - Officer Rudolph
- Matreya Scarrwener - Brittney
- Tanya Champoux - Woman
- Miranda Edwards - Coach
- Natalie Sharp - Shona
- Jess McLeod - Shelley
- Zenia Marshall - Kim
- Rowland Pidlubny - Scab
- Melanie Chan - Survivor
- Trevor Carroll - Trafficker
- Jeff Gulka - Diminutive Man
- Adam Greydon Reid - Darius
- Paul Wight - Carnage
- Jason Griffith - Guard
- Michael Frislev - The Director
- Mark Sweatman - Combatant
- Demord Dann - Special Op #2
- Klarc Jerome Wilson - Special Op #3
- Rhonda Dent - Charlotte
- Kheon Clarke - Special Op #1
- Adam Murciano - Chained Vampire
- Dakota Daulby - Willem
- Aaron Douglas - Colonel Nicholson
- Bola Omodara - Agent #1
- Kareem Malcolm - Medic
- Jeremy Jones - Deputy #2
- Christopher Gerard - Blak Tek Guard
- Viktória Spišáková - Sorina
- Heather Doerksen - Michaela
- Dan Cade - Roberto
- Kim Coates - Count Dalibor
- Sarah Aratoová - Alexandra
- Juraj Rašla - Mehai
- Raresh DiMofte - Captain Rasto
- Jill Teed - President Archer
- Donald Sales - Jeremy
- Joshua Hinkson - Sid
- Briana Skye - Mira
- Kat Ruston - Zuma
- Christina Lewall - Claire
- Christina Jastrzembska - Mama
- Jesse Stanley - Bathory
- Jesse Stanley - Oracle
- Jen Araki - Young Abby Van Helsing
- Arpad Balogh - Delta #1
- Kyle Strauts - Dominik
- Ron Selmour - Lukas
- Rami Kahlon - Shivani
- Steve Bacic - Emmett's Dad
- Laura Mennell - Rebecca
- Missy Peregrym - Scarlett Harker
- Patricia Cullen - Valerie Seward
- Michael Denis - Ted Seward
- Sandy Robson - Daniel
- Jeffrey Ballard - Aaron
- Darcy Laurie - Hopper
- Anna Galvin - Avery
- Michael Benyaer - Stefan
- Richard Meen - Frederick
- Khamisa Wilsher - Lucretia
- Nelson Leis - Leonard
- Peter Kelamis - Pauly
- Ron Wear - DC Cop
- Jennifer Cheon - Ivory
- Macie Juiles - Callie
- Ali Liebert - Nina
- Peter Bryant - Vice President Eli Newton
- Hannah Cheramy - Dylan
- Stephen Lobo - President Davis Park
- Reese Alexander - General Lannister
- Jeff C. - Aaron
- Luvia Petersen - Sgt. Weathers
- Tim Perez - Biggs
- Andrew Nadanyi - Delta Vampire
- Christian Convery - Vampire Child #4A
- Rob 'Sluggo' Boyce - Balthazar
- Tyson Larter - Boy
- Christopher Heyerdahl - Sam
- John Reardon - Dr. Harrison (as Johnny Riordan)
- John Reardon - Dr. Harrison
- Sergio Osuna - Private Mendoza
- Kimani Ray Smith
- Lynne Stopkewich
- David Frazee
- David Winning
- Simon Barry
- Jacquie Gould
- Gorrman Lee
- Jackie May
- Matt Venables
- Jeremy Smith
- Shevon Singh
- Sarah Jane Trohimchuck
- Neil LaBute
Neal McDonough hace el papel de Hansen.
Kelly Overton interpreta a Vanessa Van Helsing en la serie.
Rukiya Bernard hace el papel de Doc.
Aleks Paunovic hace el papel de Julius.
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