- Clark Gregg - Phil Coulson (voice)
- Caitlyn Taylor Love - Ava Ayala / White Tiger (voice)
- Tom Kenny - Dr. Otto Octavius / Bentley Wittman (voice)
- Dante Basco - Flash Thompson (voice)
- Chi McBride - Nick Fury (voice)
- Greg Cipes - Danny Rand / Iron Fist (voice)
- Matt Lanter - Harry Osborn (voice)
- Logan Miller - Sam Alexander / Nova (voice)
- Ogie Banks - Luke Cage / Power Man (voice)
- Ross Lynch - Jack Russell / Werewolf by Night (voice)
- Cam Clarke - Captain Ultra/Piledriver (voice)
- Mark Hamill - Arnim Zola (voice)
- Mark Hamill - Nightmare / Ox (voice)
- Mark Hamill - Nightmare / Shao Lao (voice)
- James Marsters - Korvac/Chitauri #3 (voice)
- Billy West - Rocket Raccoon (voice)
- Robert Clotworthy - Captain George Stacy (voice)
- Max Mittelman - Alex O'Hirn / Rhino (voice)
- Imari Williams - Bone Spider/Goliath Spider (voice)
- Steve Blum - Wolverine (voice)
- Peter Lurie - Sabretooth/Construction Worker/Announcer (voice)
- Grant George - Scott Lang / Ant-Man (voice)
- Debby Ryan - Jessie Prescott (voice)
- Peyton List - Emma Ross (voice)
- Cameron Boyce - Luke Ross (voice)
- Karan Brar - Ravi Ross (voice)
- Skai Jackson - Zuri Ross (voice)
- Milo Ventimiglia - Spider-Man Noir (voice)
- Seth Green - Rick Jones / A-Bombardier (voice)
- Ashley Eckstein - Dagger (voice)
- Dove Cameron - Gwen Stacy, Spider-Gwen (voice)
- Christopher Daniel Barnes - Wolf Spider, Spyder-Knight (voice)
- Cree Summer - Madame Web (voice)
- Ben Diskin - Blood Spider (voice)
- Alan Fine
- Joe Quesada
- Dan Buckley
- Leanne Moreau
- Jeph Loeb
- Stan Lee
- Kevin Manthei
Mark Hamill interpreta a Nightmare / Ox (voice) en la serie.
Drake Bell hace el papel de Peter Parker / Spider-Man (voice).
Misty Lee hace el papel de Aunt May Parker (voice).
J.K. Simmons interpreta a J. Jonah Jameson (voice) en la serie.
Chi McBride interpreta a Nick Fury (voice) en la serie.
Ogie Banks hace el papel de Luke Cage / Power Man (voice).
Capítulo disponible en las siguientes plataformas:
