- James Marshall - James Hurley
- Kyle MacLachlan - Dale Cooper
- Kyle MacLachlan - Dougie Jones / Dale Cooper
- Everett McGill - Big Ed Hurley
- Harry Goaz - Andy Brennan
- Alicia Witt - Gersten Hayward
- Michael Horse - Tommy 'Hawk' Hill
- Russ Tamblyn - Dr. Jacoby
- Wendy Robie - Nadine Hurley
- David Lynch - Gordon Cole
- Grace Zabriskie - Sarah Palmer
- Grace Zabriskie - Sarah Palmer (voice) (archive footage)
- Jack Nance - Pete Martell
- Jack Nance - Pete Martell (archive footage)
- Ray Wise - Leland Palmer
- Ray Wise - Leland Palmer (archive footage)
- Joan Chen - Jocelyn Packard
- Joan Chen - Josie Packard (archive footage)
- Al Strobel - One-Armed Man
- Al Strobel - Phillip Gerard
- Mädchen Amick - Shelly Johnson
- Mädchen Amick - Shelly
- Mädchen Amick - Shelly Briggs
- Kimmy Robertson - Lucy Moran
- Kimmy Robertson - Lucy Brennan
- Kimmy Robertson - Lucy Brennan (voice)
- Eric DaRe - Leo Johnson
- Eric DaRe - Leo Johnson (archive footage)
- Frances Bay - Mrs. Tremond
- Ritch Brinkley - D.A. Daryl Lodwick
- Clive Rosengren - Mr. Zipper
- Will Seltzer - Mr. Brunston
- Betsy Lynn George - Teen Model
- Galyn Görg - Nancy
- Austin Jack Lynch - Little Boy
- Royal Dano - Judge Clinton Sternwood
- Van Dyke Parks - Jack Racine
- Kathleen Wilhoite - Gwen Morton
- Mae Williams - Mrs. Tremond
- Gavan O'Herlihy - Officer Preston King
- Joshua Harris - Nicky
- Kenneth Welsh - Windom Earle
- Willie Garson - Heavy Metal Roadie
- Ian Buchanan - Dick Tremayne
- Mark Lowenthal - Walter Neff
- Claire Stansfield - Sid
- Ian Abercrombie - Tom Brockman
- J. Marvin Campbell - M.P. #1
- Julie Hayek - Model
- Lenny Von Dohlen - Harold Smith
- Royce D. Applegate - Reverend Clarence Brocklehurst
- Chris Mulkey - Hank Jennings
- Clarence Williams III - FBI Agent Roger Hardy
- Ron Taylor - Coach Wingate
- Nicholas Love - Malcolm Sloan
- Brenda Strong - Jones
- Jack McGee - Bartender
- David L. Lander - Tim Pinkle
- James Booth - Ernie Niles
- Michael Parks - Jean Renault
- David Warner - Thomas Eckhardt
- Tony Burton - Colonel Riley
- Jane Greer - Vivian Smythe Niles
- Jennifer Aquino - Eolani Jacoby
- Don Amendolia - Emory Battis
- Jed Mills - Wilson Mooney
- Hank Worden - Waiter
- Tony Jay - Dougie Milford
- Robert Apisa - Bodyguard on Stairs
- Dan O'Herlihy - Andrew Packard
- Robyn Lively - Lana
- Heather Graham - Annie Blackburn
- Jimmy Scott - SInger
- John Boylan - Dwayne Milford
- Charlie Spradling - Swabbie
- Mary Stavin - Heba
- Mary Jo Deschanel - Eileen Hayward
- Sheryl Lee - Laura Palmer / Maddy Ferguson
- Sheryl Lee - Maddy Ferguson
- Sheryl Lee - Laura Palmer
- Sheryl Lee - Laura Palmer (archive footage)
- Sheryl Lee - Laura Palmer / Carrie Page
- Eve Brent - Theodora Ridgely
- Troy Evans - George Wolchezk
- Lisa Ann Cabasa - Jenny
- Victoria Catlin - Blackie O'Reilly
- Michael Cera - Wally Brando
- David Duchovny - Denise Bryson
- Molly Shannon - Judy Swain
- Richard Chamberlain - Bill Kennedy
- Ben Rosenfield - Sam Colby
- Nafessa Williams - Jade
- George Griffith - Ray Monroe
- Robert Davenport - Johnny Horne
- Jessica Wallenfels - Harriet Hayward
- Roberta Maguire - Maria Pulaski
- Frank Roberts - Father Hutchings
- Jane Jones - Margaret Honeycutt
- Shelley Henning - Alice Brady
- Arnie Stenseth - Sven Jorgenson
- Rick Tutor - Janek Pulaski
- Marjorie Nelson - Janice Hogan
- Ben DiGregorio - Max Hartman
- Brett Vadset - Joey
- Erika Anderson - Jade
- Erika Anderson - Emerald / Jade
- Erika Anderson - Emerald
- David Wasman - Gilman White
- Tawnya Pettiford-Wates - Dr. Shelvy
- Dorothy Roberts - Mrs. Jackson
- Diane Caldwell - Hotel Employee
- Michael J. Anderson - Man From Another Place
- Robert Bauer - Johnny Horne
- Clay Wilcox - Bernard Renault
- Lance Davis - Chet
- Peter Michael Goetz - Jared
- Brian Straub - Einar Thorson
- Rick Giolito - Montana
- Brian Straub - Einar Thorson
- Charles Hoyes - Decker
- Charles Miller - Doctor
- Mak Takano - Asian Man
- Mak Takano - Jonathan
- Jill Pierce - Ice-Bucket Girl
- Matthew Lillard - William Hastings
- Max Perlich - Hank
- James Giordano - Officer Douglas
- Christopher Murray - Officer Olson
- Michael Bisping - Guard
- James Croak - Robby
- Allen Galli - Man in Suit
- Melissa Bailey - Marjorie Green
- Cornelia Guest - Phyllis Hastings
- Nicole LaLiberte - Darya
- Madeline Zima - Tracey
- Gia Carides - Hannah
- Neil Dickson - George Bautzer
- Piper Laurie - Catherine Martell
- Piper Laurie - Katherine Martell
- Piper Laurie - Mr. Tojamura
- Piper Laurie - Tojamura / Catherine Martell (voice)
- Piper Laurie - Tojamura / Catherine Martell
- Piper Laurie - Catherine Martell (archive footage)
- Steve Baker - Jack
- Josh McDermitt - Wise Guy
- Travis Hammer - First Trooper
- Stephen Heath - Second Trooper
- Meg Foster - Cashier
- Brian T. Finney - Security Guard
- Sara Paxton - Candy Shaker
- Ethan Suplee - Bill Shaker
- Dep Kirkland - Police Chief Mike Boyd
- Sabrina S. Sutherland - Floor Attendant Jackie
- Stephen Kearin - FBI Driver
- Brett Gelman - Supervisor Burns
- Grace Victoria Cox - Charlotte
- Jane Levy - Elizabeth
- Bob Stephenson - Frank
- Bill Tangradi - Jake
- Greg Vrotsos - Gene
- Hank Harris - Prison Tech
- Bailey Chase - Detective Don Harrison
- Sean Bolger - Detailer
- Wes Brown - Darren
- Vincent Castellanos - Federico
- Sawyer Shipman - Little Boy
- Hailey Gates - Drugged-out Mother
- Jeremy Davies - Jimmy
- Tammie Baird - Lorraine
- Ronnie Gene Blevins - Tommy
- Lisa Coronado - Hit and Run Mom
- Jeremy Lindholm - Mickey
- Sharon Van Etten - Sharon Van Etten
- Jay Jee - Patrol Officer
- Juan Carlos Cantu - Officer Reynaldo
- Bellina Logan - Desk Clerk
- Bellina Logan - Female Doctor
- Elena Satine - Rhonda
- Ernie Hudson - Colonel Davis
- Karl Makinen - Randy Hollister
- Hugh Dillon - Tom Paige
- Robert Broski - Woodsman
- Robert Broski - Woodsman (archive footage)
- Andrea Hays - Heidi
- Rebecca Field - Another Mom
- Judith Drake - Nurse
- Ivy George - 5-Year-Old Girl
- Riley Lynch - Bing
- Ted Dowling - Farmer
- Carel Struycken - The Giant
- Carel Struycken - ???????
- Carel Struycken - The Fireman
- Matt Battaglia - Cop
- Matt Battaglia - Cowboy 3
- Jesse Johnson - Younger Man
- Cullen Douglas - Disc Jockey
- Joy Nash - Senorita Dido
- Walter Olkewicz - Jacques Renault
- Walter Olkewicz - Jean-Michel Renault
- Walter Olkewicz - Jacques Renault (archive footage)
- Tad Griffith - New Mexico Husband
- Xolo Mariduena - Boy (1956)
- Trent Reznor - "The" Nine Inch Nails
- Atticus Ross - "The" Nine Inch Nails
- Robin Finck - "The" Nine Inch Nails
- Alessandro Cortini - "The" Nine Inch Nails
- Erica Eynon - Experiment Model
- Erica Eynon - Experiment
- Erica Eynon - Experiment (archive footage)
- Joey Castillo - "The" Nine Inch Nails
- Adele René - Cynthia Knox
- Charlotte Stewart - Betty Briggs
- Mark Frost - Cyril Pons
- Mark Frost - TV Newscaster
- Sky Ferreira - Ella
- Karolina Wydra - Chloe
- John Billingsley - Doctor Ben
- Jan D'Arcy - Sylvia Horne
- David Dastmalchian - Pit Boss Warrick
- Heather D'Angelo - Au Revoir Simone
- Erika Spring Forster - Au Revoir Simone
- Annie Hart - Au Revoir Simone
- Erik Rondell - Johnny Horne
- Sara Sohn - Sheena
- Christophe Zajac-Denek - Ike 'The Spike' Stadtler
- Moby - Musician
- Jane Adams - Constance Talbot
- Stephanie Allynne - Soccer Mom
- Greg Mills - Paul
- Rebekah Del Rio - Herself
- Brent Briscoe - Dave Macklay
- James Morrison - Dwight Murphy
- James Morrison - Warden Dwight Murphy (voice)
- Catherine E. Coulson - Margaret Lanterman / The Log Lady
- Catherine E. Coulson - The Log Lady
- Balthazar Getty - Red
- James Grixoni - Deputy Jesse Holcomb
- Jodi Thelen - Maggie
- Jay Larson - Limo Driver
- Linas Phillips - Russ
- Linda Porter - Lady Slot-Addict
- Lauren Tewes - Neighbor
- Marvin Rosand - Toad
- Laura Kenny - Woman in Car
- Bérénice Marlohe - French Woman
- Ashley Judd - Beverly Paige
- Ana de la Reguera - Natalie
- Elizabeth Anweis - Abbie
- Luke Judy - Warden Murphy's Son
- Scott Coffey - Trick
- Sarah Jean Long - Miriam Sullivan
- Zoe McLane - Check-out Girl
- Tom Sizemore - Anthony Sinclair
- Derek Mears - Renzo
- David Koechner - D. Fusco
- Eric Edelstein - 'Smiley' Fusco
- Larry Clarke - T. Fusco
- Frank Collison - Muddy
- John Savage - Detective Clark
- Amanda Seyfried - Rebecca "Becky" Burnett
- Christopher Durbin - The Farm Accountant
- Alon Aboutboul - Head Mover
- Kate Alden - RR Diner Waitress
- Mike Malone - Man in Urinal
- Josh Fadem - Phil Bisby
- Josh Fadem - Phil Bisby (voice)
- Virginia Kull - Szymon Waitress
- Kelsey Bohlen - Backup Singer #2
- Rachael Bower - Backup Singer #3
- Johnny Chavez - Crooked Partner
- Monica Bellucci - Monica Bellucci
- Shane Lynch - Megan
- John Paulsen - Trucker
- Emily Stofle - Sophie
- Patrick Fischler - Duncan Todd
- Caleb Landry Jones - Steven Burnett
- Harry Dean Stanton - Carl Rodd
- Joe Adler - Roger
- Grant Goodeve - Walter Lawford
- Rodney Rowland - Chuck
- Casey O'Neill - Skipper
- Jessica Szohr - Renee
- Finn Andrews - The Veils
- Sophia Burn - The Veils
- Phoebe Augustine - Ronette Pulaski
- Phoebe Augustine - American Girl
- Phoebe Augustine - Ronette Pulaski (archive footage)
- Jennifer Jason Leigh - Chantal Hutchens
- Tim Roth - Gary "Hutch" Hutchens
- David Bowie - Phillip Jeffries (archive footage)
- Jonny Coyne - Polish Accountant
- David Patrick Kelly - Jerry Horne
- Eamon Farren - Richard Horne
- Clark Middleton - Charlie
- Owain Rhys Davies - Agent Wilson
- Miguel Ferrer - Albert Rosenfield
- Miguel Ferrer - FBI Agent Albert Rosenfield (archive footage)
- J.R. Starr - MC
- Richard Bucher - Man Hit with Bottle
- Chrysta Bell - Tammy Preston
- Pierce Gagnon - Sonny Jim Jones
- Pierce Gagnon - Sonny Jim Jones (voice)
- ???? - Naido
- Robert Forster - Frank Truman
- John Pirruccello - Deputy Chad Broxford
- Jim Belushi - Bradley Mitchum
- Robert Knepper - Rodney Mitchum
- Don Murray - Bushnell Mullins
- Amy Shiels - Candie
- Giselle DaMier - Sandie
- Andrea Watrouse - Mandie
- Jay Aaseng - Drunk
- Jay R. Ferguson - Special Agent Randall Headley
- Carlton Lee Russell - Jumping Man
- Eddie Vedder - Edward Louis Severson III
- Frank Silva - Bob
- Frank Silva - Bob (archive footage)
- Laura Dern - Diane Evans
- Julee Cruise - Singer
- Julee Cruise - Julee Cruise
- Naomi Watts - Janey-E Jones
- Don S. Davis - Garland Briggs
- Don S. Davis - Major Garland Briggs (archive footage)
- Francesca Eastwood - Texas Waitress Kristi
- Rodney Harvey - Biker Scotty
- Gary Hershberger - Mike 'Snake' Nelson
- Heath Hensley - Cowboy 1
- Rob Mars - Cowboy 2
- Tracy Phillips - Receptionist
- Billy Zane - John Justice Wheeler
- Ted Raimi - Rusty Tomasky
- John Apicella - Jeffrey Marsh
- Don Calfa - Vice Principal Greege
- Cori Glazer
- Christine Larson-Nitzsche
- Johanna Ray
- Sabrina S. Sutherland
- Krista Husar
- Kate Kelly
- Matthew Rockel
- David A. Cohen
- Kerry Dean Williams
- Luke Gibleon
- Douglas Axtell
- Ronald Eng
- Willard Overstreet
Jeremy Davies hace el papel de Jimmy.
Ethan Suplee hace el papel de Bill Shaker.
Alicia Witt hace el papel de Gersten Hayward.
Tim Roth hace el papel de Gary "Hutch" Hutchens.
Kiefer Sutherland interpreta a Sam Stanley en la serie.
Tom Sizemore interpreta a Anthony Sinclair en la serie.
Naomi Watts hace el papel de Janey-E Jones.
Billy Zane hace el papel de John Justice Wheeler.
Laura Dern hace el papel de Diane Evans.
Frank Collison interpreta a Muddy en la serie.
Capítulo disponible en las siguientes plataformas:
