- Gabrielle Ruiz - Rio Morales (voice)
- Kari Wahlgren - Helen Stacy (voice)
- Dee Bradley Baker - Trace-E (voice)
- Dee Bradley Baker - Trace-E / CAL (voice)
- John Stamos - Tony Stark / Iron Man (voice)
- Stephanie Lemelin - Francine Frye / Electro (voice)
- Thomas F. Wilson - William Baker / Sandman (voice)
- Melanie Minichino - Aunt May (voice)
- Tru Valentino - T'Challa / Black Panther (voice)
- Armen Taylor - Bruce Banner / Hulk (voice)
- Eugene Byrd - Jefferson Morales (voice)
- Justin Shenkarow - Aleksei Sytsevich / Rhino (voice)
- JP Karliak - Norman Osborn / Green Goblin (voice)
- Bindi Irwin - Isla Coralton (voice)
- Sean Giambrone - Hank Pym / Ant-Man (voice)
- Kelly Ohanian - Carolyn Trainer / Doctor Octopus (voice)
- Kelly Ohanian - Carolyn Trainer / Dr. Octopus (voice)
- Maya Tuttle - Janet Van Dyne / Wasp (voice)
- Scott Porter - George Stacy (voice)
- Melique Berger - Mayor (voice)
- John Eric Bentley - Mr. Von Carnegie (voice)
- Patrick Stump - Concert Announcer (voice)
- Hayden Bishop - Jerry (voice)
- Chris Moreno
- Mitchell Stookey
- Darren Bachynski
- Harrison Wilcox
- Ashley Rideout
Thomas F. Wilson interpreta a William Baker / Sandman (voice) en la serie.
Eugene Byrd hace el papel de Jefferson Morales (voice).
Benjamin Valic interpreta a Peter Parker / Spider-Man (Spidey) (voice) en la serie.
Lily Sanfelippo interpreta a Gwen Stacy / Ghost-Spider (voice) en la serie.
Jakari Fraser interpreta a Miles Morales / Spider-Man (Spin) (voice) en la serie.
Capítulo disponible en las siguientes plataformas:
