- Ed Asner - Officer Barr (voice)
- Virginia Madsen - Silver Sable (voice)
- Ethan Embry - Max Dillon / Electro (voice)
- Ethan Embry - Max Dillon (voice)
- Michael Dorn - Kraven the Hunter (voice)
- James Marsters - Sergei (voice)
- Clancy Brown - Policeman (voice)
- Clancy Brown - Raymond (voice)
- Michael Clarke Duncan - Wilson Fisk / The Kingpin (voice)
- Stan Lee - Frank Elson (voice)
- Rob Zombie - Dr. Curt Connors / The Lizard (voice)
- Gina Gershon - Shikata (voice)
- John C. McGinley - Richard Damien (voice)
- Jane Lynch - Oscorp Executive (voice)
- Tara Strong - Christina (voice)
- Devon Sawa - Flash Thompson (voice)
- Jeffrey Combs - Professor (voice)
- Jeffrey Combs - Dr. Zellner (voice)
- Karen Maruyama - Shadowy Woman / Flower Vendor (voice)
- Karen Maruyama - Head Protestor (voice)
- Jeremy Piven - Roland Gaines (voice)
- Keith David - Agent Mosely (voice)
- Kathy Griffin - Roxanne Gaines (voice)
- Jennifer Hale - Female Reporter (voice)
- Jennifer Hale - Female News Announcer (voice)
- Julie Nathanson - Sally Johnson (voice)
- Rino Romano - Muang / Drip Den guy (voice)
- Rino Romano - Doug's friend (voice)
- Keith Carradine - J. Jonah Jameson (voice)
- Eve - Cheyenne Tate / The Talon (voice)
- Stan Lee
- Steve Ditko
Kathy Griffin interpreta a Roxanne Gaines (voice) en la serie.
Michael Dorn interpreta a Kraven the Hunter (voice) en la serie.
Neil Patrick Harris hace el papel de Peter Parker / Spider-Man (voice).
Lisa Loeb hace el papel de Mary Jane Watson (voice).
Ian Ziering interpreta a Harry Osborn (voice) en la serie.
Capítulo disponible en las siguientes plataformas:
