- Nat Faxon - Captain Underpants / Mr. Krupp (voice)
- Nat Faxon - Pharaoh (voice)
- Sean Astin - Narrator (voice)
- Sean Astin - Teachertrons (voice)
- Sean Astin - Charles Cotesworth Pinckney (voice)
- Dayci Brookshire - Jessica / Dressy (voice)
- Dayci Brookshire - Scoops Blazer (voice)
- Dayci Brookshire - Messica (voice)
- Peter Hastings - Comic Narrator (voice)
- Peter Hastings - Professor Drool (voice)
- Peter Hastings - Dr. Bagufwind (voice)
- Evan Kishiyama - Steve 'Gooch' Yamaguchi (voice)
- Evan Kishiyama - Choog (voice)
- Jorge Diaz - Melvin / Melvinborg (voice)
- Jorge Diaz - Mr. Rected (voice)
- Jorge Diaz - Ptolemy / Mr. Rected (voice)
- Jorge Diaz - Mr. Rected / Cloggernaut (voice)
- Jorge Diaz - Snelvin Meedly (voice)
- Steve-O - Lane Junkston (voice)
- Tress MacNeille - Ms. Hurd / DJ Drowsy Drawers (voice)
- Tress MacNeille - Ms. Hurd (voice)
- Patty Mattson - Miss Anthrope (voice)
- Patty Mattson - Miss Anthrope / Clogneta (voice)
- David Koechner - Mr. Meaner (voice)
- David Koechner - Mr. Meaner / Sergeant Boxers (voice)
- David Koechner - Mr. Meaner / Flabby Flabulous (voice)
- David Koechner - Mr. Meaner / Mr. Neamer / Brainy Blabulous (voice)
- David Koechner - Dr. Will Boryew (voice)
- Maggie Wheeler - Ms. Yewh / TP Mummy (voice)
- Erica Luttrell - Erica (voice)
- Erica Luttrell - Erica / Plungerina (voice)
- Erica Luttrell - Grace Wain (voice)
- Erica Luttrell - Werica Ang (voice)
- Laraine Newman - Ms. Ribble (voice)
- Laraine Newman - Ms. Ribble / Malison Saurus (voice)
- Laraine Newman - Female Cop (voice)
- Laraine Newman - Dr. Mentor / Ms. Ribble (voice)
- Laraine Newman - Ms. Ribble / Dr. Mentor (voice)
- Laraine Newman - Ms. Ribble / Queen Zombee (voice)
- Clancy Brown - Mr. Ree / Splotch (voice)
- Clancy Brown - Mr. Ree (voice)
- Eric Bauza - Rap Talkwell (voice)
- Eric Bauza - Robius (voice)
- Kevin Michael Richardson - Diddly Saurus / The Schmoozer (voice)
- Maurice LaMarche - Tubbadump (voice)
- Kari Wahlgren - Mayor Hoppy McChapper (voice)
Nat Faxon interpreta a Captain Underpants / Mr. Krupp (voice) en la serie.
Jay Gragnani interpreta a Harold (voice) en la serie.
Ramone Hamilton interpreta a George (voice) en la serie.
Sean Astin hace el papel de Narrator (voice).
Jorge Diaz hace el papel de Melvin / Melvinborg (voice).
Dayci Brookshire interpreta a Jessica / Dressy (voice) en la serie.
Capítulo disponible en las siguientes plataformas:
