- James Urbaniak - Lemur #1 (voice)
- Lance Henriksen - Doc Sugarfoot (voice)
- John DiMaggio - Jumping Rat #1 / Jumping Rat #2 / Jumping Rat #3 (voice)
- Susanne Blakeslee - Butterfly Queen (voice)
- H. Jon Benjamin - Fred the Giant Scorpion (voice)
- Ellie Kemper - Karen (voice)
- Kari Wahlgren - Female King Julien (voice)
- Maurice LaMarche - Koto / Video Game Announcer (voice)
- Maurice LaMarche - Koto (voice)
- Maurice LaMarche - Koto / Corpse (voice)
- Kether Donohue - April (voice)
- Kether Donohue - Brosalind (voice)
- Bill Fagerbakke - King Joey (voice)
- Bill Fagerbakke - King Joey / Lazy Doug (voice)
- Bill Fagerbakke - King Joey / Hans (voice)
- Bill Fagerbakke - King Joey / Leonard / Hans (voice)
- Bill Fagerbakke - Hans / Rat DJ (voice)
- David Koechner - Nurse Phantom (voice)
- David Koechner - Rob (voice)
- David Koechner - Rob / Phantom (voice)
- David Koechner - Nurse Phantom / Butterfly Prince (voice)
- David Koechner - Nurse Phantom Rob (voice)
- Rob Paulsen - Brodney (voice)
- Rob Paulsen - Captain Ethan / Lil' Arms McGee / One Eyed Simon (voice)
- Rob Paulsen - Narrator / DJ / Recorded Voice (voice)
- Rob Paulsen - Captain Ethan / Lil' Arms McGee (voice)
- Rob Paulsen - Additional Voices
- Rob Paulsen - Brodney / Snake (voice)
- Dwight Schultz - Karl (voice)
- Dwight Schultz - Karl / Patrick (voice)
- Dwight Schultz - Karl / Karl-Gram (voice)
- Grey DeLisle - Pam (voice)
- Grey DeLisle - Pam / Eloise (voice)
- Grey DeLisle - Additional Voices
- Lance Barber - Bruce (voice)
- Max Greenfield - Kipper (voice)
- Glen Powell - Trent (voice)
- Frank Welker - Grandpa Mort (voice)
- JB Blanc - Benson / Mountain Lemur Captain (voice)
- JB Blanc - Helicopter Voice (voice)
- JB Blanc - Giant Mango / Mango (voice)
- JB Blanc - Rat Bartender (voice)
- JB Blanc - Jarsh-Jarsh (voice)
- JB Blanc - Mountain Lemur Captain (voice)
- Diedrich Bader - Abner (voice)
- Diedrich Bader - Abner / Andy (voice)
- Diedrich Bader - Andy (voice)
- Diedrich Bader - Andy / Trevor (voice)
- Henry Winkler - Uncle King Julien (voice)
- John Michael Higgins - Barty (voice)
- Anjelica Huston - Julienne (voice)
- David Krumholtz - Timo (voice)
- David Krumholtz - Timo / Mom-Bot (voice)
- David Krumholtz - Timo / Dr. Watermelon Bawkings (voice)
- Crispin Freeman - Alex (voice)
- Eric Darnell
- Tom McGrath
- Randy Dormans
- Bret Haaland
- Nick Filippi
- Mitch Watson
- Ania O'Hare
Lance Henriksen interpreta a Doc Sugarfoot (voice) en la serie.
Danny Jacobs interpreta a King Julien (voice) en la serie.
Jeff Bennett hace el papel de Horst / Hector / Willie / Sage (voice).
India de Beaufort hace el papel de Clover / Crimson (voice).
Kevin Michael Richardson hace el papel de Maurice (voice).
Andy Richter interpreta a Mort / Ted (voice) en la serie.
Debra Wilson interpreta a Masikura (voice) en la serie.
Betsy Sodaro interpreta a Xixi (voice) en la serie.
Sarah Thyre interpreta a Becca / Dorothy (voice) en la serie.
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