- Monica Lewis - Official (segment "The Different Ones")
- E.G. Marshall - Jared Soames (segment "Death in the Family")
- Donald Moffat - Uncle George (segment "Pickman's Model")
- Fritz Weaver - Dr. Mazi
- Roger Davis - George Beaumont (segment "You Can Come Up Now, Mrs. Millikan")
- Kathryn Hays - June
- Chuck Connors - Roderick Blanco
- Dean Stockwell - Charlie Evans
- Barry Sullivan - Dr. Simsich
- Ellen Corby - Miss Patience
- Fay Spain - Molly Mitchell
- Lou Antonio - Jake
- Laurence Harvey - Steven Macy (segment “The Caterpillar”)
- Steve Forrest - Grant Wilson (segment "Hatred Unto Death") (as Stephen Forrest)
- Bradford Dillman - Richard Upton Pickman (segment "Pickman's Model")
- Dana Andrews - Paul Koch (segment "The Different Ones")
- Edward G. Robinson - Abraham Goldman
- Dina Merrill - Ruth Wilson (segment "Hatred Unto Death")
- Brooke Bundy - Phyllis
- Barbara Anderson - Leona Ogilvy
- Sally Field - Irene Evans
- Burl Ives - Old Man Doubleday
- Lorraine Gary - Barbara Morgan
- Gary Lockwood - James Figg
- Barbara Rush - Agatha Howard
- Raymond Massey - Dr. Glendon
- Lesley Ann Warren - Hyacinth (as Lesley Warren)
- Regis Cordic - Doctor (as Regis J. Cordic)
- Barbara Rhoades - Julie
- Fernando Lamas - Dr. Ramirez (segment "Hatred Unto Death")
- Joan Van Ark - Sondra Blanco (as Joan van Ark)
- Leonard Nimoy - Henry Auden
- Cameron Mitchell - Pete Tuttle
- Stuart Whitman - Tom Ogilvy
- Ralph Manza - Max
- Mickey Rooney - August Kolodney
- Victor Sen Yung - Joseph the Butler
- Steve Lawrence - Mark Bennett aka 'Radha Ramadi' (segment "The Dear Departed")
- Deidre Hall - Blonde (segment "An Act of Chivalry") (as Deidre Hudson)
- Rose Hobart - Mrs. Hugo (segment "The Dear Departed")
- Richard Deacon - Man with Mallet (segment "How to Cure the Common Vampire")
- Cesare Danova - Man (segment "Smile, Please")
- Johnny Brown - Man with Stake (segment "How to Cure the Common Vampire")
- Stanley Waxman - Horace Harcourt (segment "The Dear Departed")
- Elizabeth Thompson - Skinner's Woman
- Bob Crane - Ellis Travers (segment "House - With Ghost")
- Alan Napier - Cousin Zachariah Ogilvy
- Alan Napier - Doctor (segment "House - With Ghost")
- Ellen Weston - Dr. Peterson (segment "The Boy Who Predicted Earthquakes")
- Jared Martin - Tony Bolt / David Blessington (segment "Tell David...")
- Louise Sorel - Mavis Goldsmith (segment "Pickman's Model")
- Louise Sorel - Velia Redford (segment "The Dead Man")
- Imogene Coca - Wife (segment "The Merciful")
- Zara Cully - Emba (segment "Logoda's Heads")
- Denise Nicholas - Kyro (segment "Logoda's Heads")
- Sandra Dee - Ann Bolt (segment "Tell David...")
- Ross Martin - Bradley Meredith
- Rance Howard - Cameraman (segment "The Boy Who Predicted Earthquakes")
- King Donovan - Husband (segment "The Merciful")
- Harry Morgan - Thaddeus Conway
- Leif Erickson - Charlie Wheatland
- John Williams - Doctor (segment “The Caterpillar”)
- John Williams - Colonel Hymber Masters (segment "The Doll")
- Clint Howard - Herbie Bittman (segment "The Boy Who Predicted Earthquakes")
- Brock Peters - Logoda (segment "Logoda's Heads")
- James Farentino - David Faulkner
- James Farentino - Craig Lowell
- Brandon De Wilde - Johnson
- Forrest Tucker - Dr. Ernest Stringfellow
- Patrick Macnee - Major Crosby (segment "Logoda's Heads")
- Randolph Mantooth - Elkins (segment "Class of '99")
- Orson Welles - Narrator
- Tim Matheson - Henley (segment "Logoda's Heads")
- Mark Hamill - Francis, the Messenger
- Pat O'Hara - Frankie
- Ruth Buzzi - Hungry Witch (segment "Witches' Feast")
- Larry Hagman - Cedric Acton (segment "The Housekeeper")
- Suzy Parker - Carlotta Acton / Miss Wattle (segment "The Housekeeper")
- Cathleen Cordell - Miss Beamish (segment "The Housekeeper")
- Howard Morton - Headwaiter (segment "The Housekeeper")
- Carl Betz - Dr. Max Redford (segment "The Dead Man")
- Jeff Corey - Dr. Miles Talmadge (segment "The Dead Man")
- Michael Blodgett - John Michael Fearing (segment "The Dead Man")
- Glenn Dixon - Minister (segment "The Dead Man")
- Joseph Wiseman - Jacob Bauman (segment "Room with a View")
- Diane Keaton - Nurse Frances Nevins (segment "Room with a View")
- Angel Tompkins - Lila Bauman (segment "Room with a View")
- Morgan Farley - Charles (segment "Room with a View")
- John Astin - Munsch
- John Astin - Jonathan (segment "Pamela's Voice")
- Bill Quinn - Dr. Tom
- Larry Watson - Vic (segment "Room with a View")
- Burgess Meredith - Charlie Finnegan
- Chill Wills - Heppelwhite (segment "The Little Black Bag")
- George Furth - Gillings (segment "The Little Black Bag")
- E.J. André - Charlie Peterson (segment "The Little Black Bag")
- Agnes Moorehead - Emma Brigham (segment "Certain Shadows on the Wall")
- Agnes Moorehead - Head Witch (segment "Witches' Feast")
- Arthur Malet - Ennis (segment "The Little Black Bag")
- Eunice Suarez - Puerto Rican Mother (segment "The Little Black Bag")
- Johnny Silver - Pawnbroker (segment "The Little Black Bag")
- C. Lindsay Workman - 1st Doctor (segment "The Little Black Bag") (as Lindsay Workman)
- Matt Pelto - 2nd Doctor (segment "The Little Black Bag")
- Ralph Moody - Jake (segment "The Little Black Bag")
- William Challee - 2nd Old Man (segment "The Little Black Bag")
- Joseph Campanella - Simms (segment "The Nature of the Enemy")
- Richard Van Vleet - Commander Perkins (segment "The Nature of the Enemy")
- Geraldine Page - Molly Wheatland
- Geraldine Page - Mrs. Evans
- Jason Wingreen - 2nd Reporter (segment "The Nature of the Enemy")
- Albert Popwell - 3rd Reporter (segment "The Nature of the Enemy")
- Jerry Strickler - Man (segment "The Nature of the Enemy")
- Lindsay Wagner - Girl (segment "Smile, Please")
- Desi Arnaz Jr. - Doran (segment "Death in the Family")
- Steve Franken - Dr. Peter Mitchell (segment "The House")
- Louis Hayward - Dr. Stephen Brigham (segment "Certain Shadows on the Wall")
- Grayson Hall - Ann Brigham (segment "Certain Shadows on the Wall")
- Rachel Roberts - Rebecca Brigham (segment "Certain Shadows on the Wall")
- Jan Burrell - Nurse (segment "The House")
- Almira Sessions - Old Woman (segment "The House")
- Godfrey Cambridge - Jackie Slater (segment "Make Me Laugh")
- Tom Bosley - Jules Kettleman (segment "Make Me Laugh")
- Jackie Vernon - Chatterje (segment "Make Me Laugh")
- Tom Troupe - Jeffrey Pierce (segment "Clean Kills and Other Trophies")
- Barry Brown - Archie Dittman Jr. (segment "Clean Kills and Other Trophies")
- Herbert Jefferson Jr. - Tom Mboya (segment "Clean Kills and Other Trophies")
- Jeanette Nolan - Aunt Ada Burn Quigley
- Jeanette Nolan - Miss Wattle / Carlotta Acton (segment "The Housekeeper")
- Al Lewis - Mishkin (segment "Make Me Laugh")
- Sidney Clute - David Garrick (segment "Make Me Laugh")
- John J. Fox - Heckler (segment "Make Me Laugh")
- Gene R. Kearney - 2nd Bartender (segment "Make Me Laugh") (as Gene Kearney)
- Tony Russel - Director (segment "Make Me Laugh")
- Sonny Klein - 1st Bartender (segment "Make Me Laugh")
- Michele Hart - Miss Wilson (segment "Make Me Laugh") (as Michael Hart)
- Georgia Schmidt - Flower Lady (segment "Make Me Laugh")
- Phyllis Diller - Pamela (segment "Pamela's Voice")
- John Colicos - Survivor (segment "Lone Survivor")
- Torin Thatcher - Captain of the Lusitania (segment "Lone Survivor")
- Hedley Mattingly - Doctor on the Lusitania (segment "Lone Survivor")
- Shani Wallis - Miss Danton (segment "The Doll")
- Henry Silva - Pandit Chola (segment "The Doll")
- Brendan Dillon - Quartermaster - Lusitania (segment "Lone Survivor")
- William Beckley - Richards - Lusitania (segment "Lone Survivor")
- Edward Colmans - Captain - Andrea Dorea (segment "Lone Survivor")
- Pierre Jalbert - Officer of Watch - Andrea Dorea (segment "Lone Survivor")
- Carl Milletaire - Quartermaster - Andrea Dorea (segment "Lone Survivor")
- Diane Baker - Lynn Alcott (segment "They're Tearing Down Tim Riley's Bar")
- Bert Convy - Harvey Doane (segment "They're Tearing Down Tim Riley's Bar")
- John Randolph - H.E. Pritkin (segment "They're Tearing Down Tim Riley's Bar")
- Jack Cassidy - Marius Davis (segment "The Last Laurel")
- Martine Beswick - Susan Davis (segment "The Last Laurel")
- Henry Beckman - The Policeman (segment "They're Tearing Down Tim Riley's Bar")
- James B. Sikking - 1st Reporter (segment "The Nature of the Enemy")
- James B. Sikking - State Trooper (segment "Death in the Family")
- David Astor - Blodgett (segment "They're Tearing Down Tim Riley's Bar")
- Gene O'Donnell - Bartender (segment "They're Tearing Down Tim Riley's Bar")
- Frederic Downs - Father (segment "They're Tearing Down Tim Riley's Bar")
- Mary Gail Hobbs - Miss Trevor (segment "They're Tearing Down Tim Riley's Bar")
- Martin E. Brooks - Doctor Armstrong (segment "The Last Laurel")
- Jonathan Harris - Prof. Nicholas Porteus
- Michele Lee - Joanna Lowell
- David McCallum - Dr. Joel Winter
- Lonny Chapman - Father
- Linda Marsh - Mildred Squire
- Joanna Pettet - Rhona Warwick (segment “The Caterpillar”)
- Joanna Pettet - The Girl with the Hungry Eyes
- Joanna Pettet - Elaine Latimer (segment "The House")
- David Carradine - Gideon
- Ford Rainey - The Sheriff
- Jack Bannon - Al
- Alex Cord - Erik Sutton
- Michael Baseleon - Tom Robb
- Richard Kiley - Ralph Burke
- Jill Ireland - Ann Loring
- Joel Grey - Andrew MacBane
- Royal Dano - Owen
- John Saxon - Ianto
- Darrell Larson - Elie Green
- Richard Thomas - Ian Evans
- Michael Dunn - The Servant
- Cloris Leachman - Mrs. Fulton
- Broderick Crawford - Joseph Fulton
- Lana Wood - Maid
- Barbara Steele - The Widow Craighill
- Ed Nelson - Tom Burke (segment “Little Girl Lost”)
- Tisha Sterling - Fern
- Leslie Nielsen - The Phantom (segment "Phantom of What Opera?")
- Leslie Nielsen - Col. Dennis Malloy
- Kip Niven - Harry Krell
- Charles Davis - Officer of Watch - Lusitania (segment "Lone Survivor")
- Trisha Noble - Sherry (segment "House - With Ghost")
- William Windom - Professor Putman (segment “Little Girl Lost”)
- Ivor Francis - Dr. Charles Cottrell (segment “Little Girl Lost”)
- Tom Helmore - John Warwick (segment “The Caterpillar”)
- Harriet Nelson - Helena Millikan (segment "You Can Come Up Now, Mrs. Millikan")
- Ozzie Nelson - Henry Millikan (segment "You Can Come Up Now, Mrs. Millikan")
- Michael Lerner - Dr. Burgess (segment "You Can Come Up Now, Mrs. Millikan")
- Henry Jones - Malcolm Hample
- Cyril Delevanti - The Old Man
- Michael Constantine - Wellman (segment "The Boy Who Predicted Earthquakes")
- Sue Lyon - Betsy (segment "Miss Lovecraft Sent Me")
- George Maharis - Peter Lacland (segment "The Hand of Borgus Weems")
- Kent Smith - Bennett
- Kent Smith - Doctor
- Bernie Kopell - Reed (segment "The Boy Who Predicted Earthquakes")
- Gene Tyburn - Floor Director (segment "The Boy Who Predicted Earthquakes")
- Larry D. Mann - Hannify
- Peter Brocco - Dr. Shockman
- Marjorie Bennett - Mrs. Woods
- John Lasell - Colonel Hawes (segment "Little Girl Lost")
- Don Knight - Tommy Robinson (segment "The Caterpillar")
- Sandy Ward - Irate Man (segment "Little Girl Lost") (uncredited)
- Kenneth Tobey - Warden
- Roger E. Mosley - Prisoner
- Raymond Mayo - Prisoner
- Marty Allen - Edgar Allan Poe
- Henry Darrow - Dr. Juan Munos
- Larry J. Blake - Charles Crowley
- Mel Blanc - Raven (voice) (uncredited)
- Norman Lloyd - Henry Mallory
- John Alderson - Granger
- Hermione Baddeley - Mrs. Gray
- Adam West - Mr. Hyde
- Arte Johnson - Craig Lowell
- Charles Seel - Cemetery Caretaker
- Lisabeth Hush - Mother
- Radames Pera - Paul
- Ray Ballard - Mr. Grouch
- Herbert Wright
- Paul Freeman
- John Badham
- Jack Laird
Mark Hamill interpreta a Francis, the Messenger en la serie.
Diane Keaton interpreta a Nurse Frances Nevins (segment "Room with a View") en la serie.
Sally Field interpreta a Irene Evans en la serie.
Brock Peters hace el papel de Logoda (segment "Logoda's Heads").
Raymond Massey interpreta a Dr. Glendon en la serie.
Norman Lloyd interpreta a Henry Mallory en la serie.
David Carradine interpreta a Gideon en la serie.
Mickey Rooney interpreta a August Kolodney en la serie.
Michael Lerner hace el papel de Dr. Burgess (segment "You Can Come Up Now, Mrs. Millikan").
Dean Stockwell interpreta a Charlie Evans en la serie.
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