- Ed Asner - Ben Parker (voice)
- Grey DeLisle - Sally Avril (voice)
- Grey DeLisle - Betty Brant (voice)
- Grey DeLisle - Sally Avril / Betty Brant (voice)
- Grey DeLisle - Sally Avril / Coffee Clerk (voice)
- Grey DeLisle - Sally Avril / Computer Voice (voice)
- Grey DeLisle - Sally Avril / Voice of OsCorp (voice)
- Grey DeLisle - Sally Avril / Bette Brant (voice)
- Grey DeLisle - Stephanie Briggs (voice)
- Grey DeLisle - Voice of OsCorp (voice)
- Grey DeLisle - Sally Avril / Erin (voice)
- Phil LaMarr - Rand Robertson (voice)
- Phil LaMarr - Rand Robertson / Robbie Robertson (voice)
- Phil LaMarr - Robbie Robertson (voice)
- Phil LaMarr - Fancy Dan / Ricochet / Rand Robertson (voice)
- Phil LaMarr - Rand Robertson / Fancy Dan (voice)
- Phil LaMarr - Fancy Dan / Homunculus #1 (voice)
- Cree Summer - Glory Grant (voice)
- Cree Summer - Glory Grant / Cobweb / Homunculus #2 (voice)
- Kevin Michael Richardson - Tombstone (voice)
- Kevin Michael Richardson - Tombstone / Principal Davis (voice)
- Kevin Michael Richardson - Principal Davis (voice)
- Kevin Michael Richardson - Coach Smith (voice)
- Kevin Michael Richardson - Big Man / Tombstone / L. Thompson Lincoln (voice)
- Kevin Michael Richardson - Big Man / Coach Smith / Thug (voice)
- Kevin Michael Richardson - OsCorp Security Guard (voice)
- Tricia Helfer - Black Cat (voice)
- Tricia Helfer - Felicia Hardy / Black Cat (voice)
- Robert Englund - Vulture (voice)
- Robert Englund - Adrian Toomes / Vulture (voice)
- Crispin Freeman - Electro (voice)
- Crispin Freeman - Thug #1 (voice)
- Crispin Freeman - Max Dillon / Electro (voice)
- Crispin Freeman - Electro / Clerk (voice)
- Peter MacNicol - Otto Octavius (voice)
- Peter MacNicol - Doctor Octopus (voice)
- Peter MacNicol - Master Planner (voice)
- Peter MacNicol - Master Planner / Otto Octavius (voice)
- Peter MacNicol - Master Planner / Doctor Octopus (voice)
- Peter MacNicol - Otto Octavius / Doctor Octopus (voice)
- Deborah Strang - May Parker (voice)
- Dee Bradley Baker - Curt Connors (voice)
- Dee Bradley Baker - Curt Connors / Lizard (voice)
- Dee Bradley Baker - Curt Connors / Homunculi (voice)
- John DiMaggio - Hammerhead (voice)
- John DiMaggio - Sandman / Hammerhead (voice)
- John DiMaggio - Flint Marko / Hammerhead (voice)
- John DiMaggio - Hammerhead / Car Thief #2 (voice)
- John DiMaggio - Sandman (voice)
- John DiMaggio - Flint Marko / Sandman / Hammerhead (voice)
- Ben Diskin - Eddie Brock (voice)
- Ben Diskin - Venom (voice)
- Ben Diskin - Eddie Brock / Venom (voice)
- Clancy Brown - George Stacy (voice)
- Clancy Brown - Rhino / George Stacy (voice)
- Clancy Brown - Alex O'Hirn (voice)
- Clancy Brown - Alex O'Hirn / Ox (voice)
- Clancy Brown - Alex O'Hirn / Rhino / George Stacy (voice)
- Joshua LeBar - Flash Thompson (voice)
- Joshua LeBar - Flash Thompson / Car Thief #1 (voice)
- Vanessa Marshall - Mary Jane Watson (voice)
- Vanessa Marshall - Mary Jane Watson / Rosie Thompson (voice)
- Vanessa Marshall - Mary Jane Watson / Hermia (voice)
- Daran Norris - J. Jonah Jameson (voice)
- Daran Norris - J. Jonah Jameson / John Jameson (voice)
- Daran Norris - J. Jonah Jameson / John Jameson / Colonel Jupiter (voice)
- James Arnold Taylor - Harry Osborn (voice)
- James Arnold Taylor - Harry Osborn / Frederick Foswell (voice)
- James Arnold Taylor - Harry Osborn / Alan O'Neil (voice)
- James Arnold Taylor - Harry Osborn / Patch / Frederick Foswell (voice)
- James Arnold Taylor - Patch / Homunculi (voice)
- Alan Rachins - Norman Osborn (voice)
- Alanna Ubach - Liz Allan (voice)
- Alanna Ubach - Liz Allan / Trina (voice)
- Alanna Ubach - Liz Allan / Helena (voice)
- Lacey Chabert - Gwen Stacy (voice)
- Miguel Ferrer - Silvermane (voice)
- Miguel Ferrer - Silvio Manfredi / Silvermane (voice)
- Danny Trejo - Ox (voice)
- Stan Lee - Stan (voice)
- Eric Vesbit - Sergei Kravinoff / Kraven the Hunter (voice)
- Eric Vesbit - Kraven (voice)
Capítulo disponible en las siguientes plataformas:
