- Matt Keeslar - Richard Connell
- Gregg Henry - William Bashford
- Octavia Spencer - Professor Janack
- Glenn Morshower - Sheriff Rand
- Roy Vongtama - Attendant #4
- Jamie Bamber - Martin Klar
- Alan Tudyk - Stephen Kepler / Alpha
- Summer Glau - Bennett Halverson
- Keith Carradine - Matthew Harding
- Vincent Laresca - Mr. Sunshine
- Josh Cooke - Leo Carpenter
- Tim Conlon - Shaw
- Ian Anthony Dale - Jack Dunston
- Jillian Armenante - Grace
- Liza Lapira - Ivy
- Ana Claudia Talancón - Gelena
- Vincent Ventresca - Nolan Kinnard
- Haley Pullos - Davina Crestejo
- Adam Godley - Clyde Randolph
- Clyde Kusatsu - Dr. Makido
- Teddy Sears - Mike
- Jordan Bridges - Nicholas Bashford
- Jeff Doucette - Store Manager
- Philip Casnoff - Clive Ambrose
- Stacey Scowley - Cindy Perrin
- Alexis Denisof - Senator Daniel Perrin
- Ashley Johnson - Wendy / Caroline
- David Lee Smith - Clay Corman
- Kristoffer Polaha - Nate Jordan
- Jenica Bergere - Jessie
- Reed Diamond - Laurence Dominic
- Mehcad Brooks - Sam Jennings
- Rick Otto - Gabe
- Ryan Alosio - Lars
- Jonathan Del Arco - Mr. Caviezel
- Brett Claywell - Matt Cargill
- Zack Ward - Zone
- Kurt Caceres - Gabriel Crestejo
- Tim Kelleher - Detmer
- Michael Hogan - Bradley Karrens
- Nate Dushku - Clive Ambrose
- Katie Chonacas - Nikole
- Joseph Sikora - Terry Karrens
- Matt Winston - Franklin
- Aisha Hinds - Loomis
- Arye Gross - Professor Gossen
- Susan Ziegler - Mother
- Danielle Langlois - Aunt Sheila
- Tara Holt - Big Sis
- Ray Wise - Howard Lipman
- Mark Sheppard - Tanaka
- Rhea Seehorn - Jocelyn Bashford
- Ido Mor - Talik Raj Amin
- Tony Amendola - Atalo Diakos
- Jay Malone - Clyde Randolph
- Megan Heyn - Cafe Girl #2
- Billy Snow - Test Subject #2
- Rhashan Stone - Test Subject
- Rick Fox - Gary Walden (uncredited)
- Dennis Keiffer - Ambrose's Man (uncredited)
- Mike Massa - Apollo (uncredited)
- Felicia Day - Mag
- Kevin Kilner - Joe Hearn
- Jaime Lee Kirchner - Rayna Russell
- Jim Piddock - Biz
- Graham Norris - The Fan
- Miracle Laurie - Madeline Costley
- Miracle Laurie - Mellie
- Miracle Laurie - November / Mellie
- Miracle Laurie - November / Mellie / Madeline Costley
- Mark Ivanir - Cyril
- Toby Leonard Moore - Walton
- Anson Mount - Vitas
- Andrew Bowen - Scott
- Sarah McElligott - Nancy
- David Alpay - Seth
- Brian Bloom - Jonas Sparrow
- Rebecca Field - Kris
- Sam Hennings - Senator Boxbaum
- Erin Cummings - Attendant
- Erin Cummings - Staff Member
- Mark Totty - Agent Lilly
- Brad Hunt - Jesse Dillard
- John Eddins - Male ATF Agent
- Blake Kushi - ATF Board Agent
- Keri Safran - Female ATF Agent
- Ben Bode - Academic
- Patton Oswalt - Joel Mynor
- David Barry Gray - Bicks
- Lydia Blanco Garza - Housewife
- Leslie Andrews - Female Student
- Josh Fadem - Owen Johnson
- Nick George - Trevor
- Rome Shadanloo - Ingrid
- Emma Bell - Tango
- Skyler Stone - Jimmy
- Angel Desai - Sophie Alvarez
- Taira Soo - Attendant #1
- Amelia Hoy - Attendant #2
- Vinny Chhibber - Attendant #3
- Valerie Cruz - Selena Ramirez
- Greg Collins - Older Man
- Greg Collins - Molester
- Tim Chiou - Travis Nikoden
- Paul Ganus - Handler
- Damien Leake - Minister
- Brenda Bakke - Margaret Bashford
- Hannah Leigh - Susan
- Judith Moreland - Renee
- Charles Dougherty - Man
- Marco Sanchez - Blevins
- Natalie Avital - Nita Walsh
- Joe Howard - Old Dr. Saunders
- Zoran Radanovich - Hugo Taubman
- Ben Hermes - Handler
- Jesus Ruiz - Justice
- Iris Bahr - Detective Donovan
- Sergio Enrique - Sanchez
- John Kelly - Captain
- Deanna Lee Douglas - Little Sister
- Carlease Burke - Pam
- Nelson Franklin - Burt Styne
- Maurissa Tancharoen - Kilo
- Kevin Linehan - Deputy Merrick
- Leslie Stevens - Cashier
- Damion Poitier - Yankee
- Kate Comer - Attendant
- Michael King - Hagen
- Katrina Nelson - Bartender
- Tzi Ma - Matsu
- Jeff Cole - Male Attendant #2
- Evie Peck - Female Staff Member
- Tanoai Reed - Dark Figure
- Josh Latzer - Security Guard #1
- Adair Tishler - Caroline Farrell
- Noah Harpster - Matthew Harper
- Christian Monzon - Romeo
- Tank Jones - Security Guard #2
- Billy Beck - Old Man
- Amy Acker - Dr. Claire Saunders
- Amy Acker - Dr. Claire Saunders / Whiskey
- Amy Acker - Dr. Claire Saunders / Whiskey / Crystal
- Amy Acker - Clyde Randolph
- Daniel Kash - Guard #2
- Joss Whedon
- David Solomon
- Sarah Fain
- Tim Minear
- Steven S. DeKnight
- Jane Espenson
Brian Bloom interpreta a Jonas Sparrow en la serie.
Vincent Laresca hace el papel de Mr. Sunshine.
Clyde Kusatsu interpreta a Dr. Makido en la serie.
Gregg Henry interpreta a William Bashford en la serie.
Eliza Dushku interpreta a Echo / Caroline Farrell en la serie.
Fran Kranz interpreta a Topher Brink en la serie.
Olivia Williams interpreta a Adelle DeWitt en la serie.
Dichen Lachman interpreta a Sierra en la serie.
Enver Gjokaj hace el papel de Victor.
Tahmoh Penikett interpreta a Paul Ballard en la serie.
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