- John DiMaggio - Big Brother (voice)
- Tom Kane - Simon (voice)
- Maurice LaMarche - Father (voice)
- Frank Welker - Professor XXXL (voice)
- Frank Welker - Truck Driver (voice)
- Frank Welker - Rainbow Monkey Kong (voice)
- Frank Welker - Bus Driver (voice)
- Mark Hamill - Stickybeard / Sumo Wrestler / Pirate (voice)
- Mark Hamill - Stickybeard (voice)
- Rob Paulsen - (voice)
- Rob Paulsen - Robin Food (voice)
- Rob Paulsen - Mr. Washer / Chili Dog Vendor (voice)
- Rachael MacFarlane - (voice)
- Rachael MacFarlane - Numbuh 362 (voice)
- Jason Harris - (voice)
- Jason Harris - Commander (voice)
- Jason Harris - Numbah 274 (voice)
- Jason Harris - Chad Dickson (voice)
- Josh Peck - Numbuh 50 Million B.C. (voice)
- Kevin Michael Richardson - Dr. Lincoln (voice)
- Kevin Michael Richardson - Ice Cream Man / Ice Cream Beast (voice)
- Jeff Bennett - (voice)
- Jeff Bennett - Destructo Dad / Mr. Boss / Mr. Fizz (voice)
- Jeff Bennett - Mr. Boss (voice)
- Jeff Bennett - Hoagie Gilligan Sr. (voice)
- Jeff Bennett - Numbuh Jebediah (voice)
- Jeff Bennett - Sydney Beetles (voice)
- James Arnold Taylor - Eggbert Eggleston (voice)
- James Arnold Taylor - King Sandy / Knight #2 / Guard #1 (voice)
- Keone Young - Kani Sanban (voice)
- Daran Norris - (voice)
- Daran Norris - Joe Balooka (voice)
- Daran Norris - Ed / Guard #1 / Lead Mustache (voice)
- Daran Norris - Mr. Physically Fitastic / The Human Text (voice)
- Daran Norris - RAMON-4 (voice)
- Daran Norris - Count Spankulot / Gooey / Ice Cream Man #2 (voice)
- Daran Norris - Count Spankulot / Joe Balooka / Spankenstine Monster (voice)
- Daran Norris - Gooey (voice)
- Daran Norris - Maitre'd Violinist (voice)
- Grey DeLisle - Lizzie Divine (voice)
- Grey DeLisle - Crazy Old Cat Lady / Mega Mom (voice)
- Grey DeLisle - Julie (voice)
- Grey DeLisle - Gramma Stuffum (voice)
- Grey DeLisle - Grace / Lizzie Devine (voice)
- Grey DeLisle - Laura Limpin (voice)
- Grey DeLisle - Mega Mom / Numbuh 66 (voice)
- Tom Kenny - (voice)
- Tom Kenny - Chester (voice)
- Tom Kenny - Narrator (voice)
- Tom Kenny - Numbuh 30C (voice)
- Tom Kenny - Hoagie Gilligan IV (voice)
- Tom Kenny - Chester / President / Robber #1 (voice)
- Tom Kenny - Numbuh 42 / Numbuh Ezekiel (voice)
- Tom Kenny - Goof (voice)
- Tom Kenny - Twin Boy / Vicious Dog (voice)
- Tom Kenny - Knightbrace (voice)
- Tom Kenny - Fake Delightful Child from Down the Lane (voice)
- Tom Kenny - Mr. Jelly / Knightbrace (voice)
- Tom Kenny - Mr. Wink (voice)
- Tom Kenny - Numbuh 30c (voice)
- Carlos Alazraqui - Numbuh 2x4 (voice)
- Carlos Alazraqui - The Kid / Pedestrian (voice)
- Jess Harnell - Tony Clownarelli (voice)
- Jason Marsden - Numbuh 80 (voice)
- Jennifer Hale - (voice)
- Jennifer Hale - Mrs. Beetles (voice)
- Jennifer Hale - Mrs. Thompson (voice)
- Jennifer Hale - Dixie / Runt (voice)
- Jennifer Hale - Numbuh 86 / Nurse Claiborne / Rainbow Monkey Chorus (voice)
- Jennifer Hale - Mrs. Thompson / Robber #3 (voice)
- Jennifer Hale - Numbuh 202 (voice)
- Jennifer Hale - Stacey (voice)
- Jennifer Hale - Mrs. Uno (voice)
- Jennifer Hale - Computer (voice)
- Dave Wittenberg - Nick (voice)
- Dave Wittenberg - Wintergreen (voice)
- Dave Wittenberg - Ernest "Ernie" / Nick (voice)
- Dave Wittenberg - Numbuh 74.239 (voice)
- Candi Milo - Betty Gilligan / Grandma Lydia (voice)
- Candi Milo - Lasso Lass (voice)
- Candi Milo - Grandma Lydia (voice)
- Candi Milo - Edna Jucation (voice)
- Candi Milo - Leona (voice)
- Candi Milo - Betty Gilligan (voice)
- Candi Milo - Nurse Jumbo (voice)
- Candi Milo - Henrietta Von Marzipan (voice)
- Candi Milo - Leaky Leona / Mrs. Goodwall (voice)
- Candi Milo - Madame Margaret (voice)
- Tara Strong - Sally Sanban (voice)
- Tara Strong - Pauley Marcus (voice)
- Tara Strong - Mushi Sanban / Numbuh 93 (voice)
- Tara Strong - Mushi Sanban (voice)
- Tara Strong - Mushi Sanban / Stewie (voice)
- Tara Strong - Rainbow Monkey Tribesgirl #3 (voice)
- Matt Levin - Hooves (voice)
- Roger Rose - Little Juan (voice)
- Roger L. Jackson - Bonko (voice)
- Hynden Walch - Numbuh Rebecca (voice)
- Thomas Chase
- Steve Rucker
Mark Hamill hace el papel de Stickybeard (voice).
Ben Diskin interpreta a Numbuh 1 / Numbuh 2 (voice) en la serie.
Lauren Tom interpreta a Numbuh 3 (voice) en la serie.
Dee Bradley Baker interpreta a Numbuh 4 (voice) en la serie.
Cree Summer interpreta a Numbuh 5 (voice) en la serie.
Capítulo disponible en las siguientes plataformas:
