- Mark Hamill - The Joker (voice)
- Brad Garrett - Goliath (voice)
- Paul Winfield - Earl Cooper (voice)
- David Warner - Ra's al Ghul (voice)
- George Murdock - Boss Biggis (voice)
- Thomas F. Wilson - Zucco (voice)
- George DiCenzo - Ubu (voice)
- Brock Peters - Lucius Fox (voice)
- Ron Perlman - Driller (voice)
- William Sanderson - Karl Rossum (voice)
- Brion James - Additional Voices (voice)
- Jeffrey Tambor - Crocker (voice)
- LeVar Burton - Hayden Sloane (voice)
- Vincent Schiavelli - John Zatara (voice)
- Katherine Helmond - Connie (voice)
- John Rhys-Davies - 'Baron' Waclaw Jozek (voice)
- Earl Boen - Rhino (voice)
- Roy Dotrice - Frederick (voice)
- Treat Williams - Milo (voice)
- Jeffrey Jones - Nivens (voice)
- Greg Burson - Mad Dog (voice)
- Hector Elizondo - Fallbrook (voice)
- Nichelle Nichols - Thoth Khepera (voice)
- Bob Kane
- Bruce Timm
- Alan Burnett
- Andrea Romano
- Jean MacCurdy
- Tom Ruegger
- Paul Dini
- Danny Elfman
Mark Hamill interpreta a The Joker (voice) en la serie.
Brad Garrett interpreta a Goliath (voice) en la serie.
Paul Winfield interpreta a Earl Cooper (voice) en la serie.
David Warner interpreta a Ra's al Ghul (voice) en la serie.
George Murdock interpreta a Boss Biggis (voice) en la serie.
Thomas F. Wilson interpreta a Zucco (voice) en la serie.
George DiCenzo hace el papel de Ubu (voice).
Brock Peters hace el papel de Lucius Fox (voice).
Ron Perlman interpreta a Driller (voice) en la serie.
William Sanderson interpreta a Karl Rossum (voice) en la serie.
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